CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 14998782

Catalog No. 50-139-71

Description

Description: The HIR2 monoclonal antibody reacts with human glycophorin A, a sialoglycoprotein expressed by erythroid precursors and mature circulating red cells. Applications Reported: HIR2 can be used in flow cytometric analysis. Applications Tested: The HIR2 (also GA-R2) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and cell agglutination assay and can be used at approximately 0.1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

CD235a (Glycophorins A, GPA) is a single pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells. CD235a is the carrier of blood group M and N specificities. CD235a provides cells with a large mucin-like that may serve as a barrier to cell fusion, minimizing aggregation between red blood cells in the circulation. CD235a has been shown to act as a receptor for Sandei virus, parvovirus, and Hsa, an Streptococcus adhesin.

Specifications

• Antigen: CD235a (Glycophorin A)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P02724
• Gene Symbols: GYPA
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 2993
• Content And Storage: 4° C
• Form: Liquid

• Applications: Flow Cytometry
• Clone: HIR2 (GA-R2)
• Conjugate: Unconjugated
• Gene: GYPA
• Gene Alias: AI853584; CD235; CD235a; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A; glycophorin A (MN blood group); glycophorin A (MNS blood group); glycophorin A, GPA; glycophorin Erik; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Sta type C; glycophorin-A; GPA; GPErik; GPSAT; Gypa; HGNC:4702; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); HGpStaC; Mi.V glycoprotein; Mi.V glycoprotein (24 AA); MN; MN sialoglycoprotein; MNS; PAS-2; recombinant glycophorin A-B Miltenberger-DR; sialoglycoprotein alpha
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b κ

For Research Use Only.