CD235a (Glycophorin A) Mouse anti-Human, PE, Clone: HIR2 (GA-R2), eBioscience
Mouse Monoclonal Antibody
Manufacturer: Invitrogen 12998780
Description: The HIR2 monoclonal antibody reacts with human glycophorin A, sialoglycoproteins expressed by erythroid precursors and mature circulating red cells. Applications Reported: The HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis. Applications Tested: The HIR2 (also GA-R2) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.015 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.Glycophorins A (GPA) and B (GPB) which are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.
|CD235a (Glycophorin A)|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|4° C, store in dark, DO NOT FREEZE!|
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