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CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), NovaFluor™ Yellow 660, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Specifications
Antigen | CD235a (Glycophorin A) |
---|---|
Clone | HIR2 (GA-R2) |
Concentration | 0.1 μg/Test |
Applications | Flow Cytometry |
Classification | Monoclonal |
Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
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Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
H050T02Y04
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LIFE TECHNOLOGIES
H050T02Y04 |
25 Tests | This item has been discontinued and is no longer available. Please call customer service for assistance: 1-800-766-7000. | N/A |
H050T03Y04
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LIFE TECHNOLOGIES
H050T03Y04 |
100 Tests | This item has been discontinued and is no longer available. Please call customer service for assistance: 1-800-766-7000. | N/A |
Description
The HIR2 monoclonal antibody reacts with human glycophorin A, a sialoglycoprotein expressed by erythroid precursors and mature circulating red cells. Applications Reported: This HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis. Applications Tested: This HIR2 (also GA-R2) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.015 μg) per test Our internal testing shows that NovaFluor Yellow 660 non-specifically stains B cells in SJL mice. Non-specific staining has not been observed in BALB/c or C57BL/6 mice. Other strains have not been tested. See the Antibody Testing Data for an example of this strain-dependent difference. NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes. Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer. Use 5 μL of CellBlox Blocking Buffer per stained cell sample containing 10^3 to 10^8 cells. NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Excitation: 552 nm; Emission: 663 nm; Laser: 561 nm (Yellow) Laser
CD235a (Glycophorin A) Monoclonal antibody specifically detects CD235a (Glycophorin A) in Human samples. It is validated for Flow CytometrySpecifications
CD235a (Glycophorin A) | |
0.1 μg/Test | |
Monoclonal | |
Liquid | |
RUO | |
PBS with 0.09% sodium azide; pH 7.2 | |
AI853584; CD235; CD235a; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A; glycophorin A (MN blood group); glycophorin A (MNS blood group); glycophorin A, GPA; glycophorin Erik; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Sta type C; glycophorin-A; GPA; GPErik; GPSAT; Gypa; HGNC:4702; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); HGpStaC; Mi.V glycoprotein; Mi.V glycoprotein (24 AA); MN; MN sialoglycoprotein; MNS; PAS-2; recombinant glycophorin A-B Miltenberger-DR; sialoglycoprotein alpha | |
IgG2b κ | |
Affinity chromatography | |
Antibody |
HIR2 (GA-R2) | |
Flow Cytometry | |
NovaFluor Yellow 660 | |
Mouse | |
Human | |
P02724 | |
2993 | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
GYPA |
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