MilliporeSigma™ CD27, Rat, Unlabeled, Clone: RM27-3E5,

Rat Monoclonal Antibody

Supplier:  MilliporeSigma™ 041475I

Catalog No. 041475I

Description

CD27 antigen (UniProt P41272; also known as CD27, CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7) is encoded by the Cd27 (also known as Tnfrsf7) gene (Gene ID 21940) in murine species. CD27 is a TNF receptor superfamily member that binds to CD70 and mediates the survival, function, and differentiation of T-, B-, natural killer (NK), and plasma cells. Interaction with CD70 provides costimulation in T cells induces signaling events essential for cell proliferation, long-term maintenance of antigen-specific T cells, antiviral responses, antitumor immunity, and alloreactivity. CD27 ligation on B-cells by CD70 causes enhanced plasma cell formation and increased IgG production. CD27 is also used as a marker for memory B cells in the classification of B-cell deficiencies, including common variable immunodeficiency (CVID). CD27 is synthesized with a signal peptide (a.a. 1-23), which was removed posttranslationally to yield the mature type I transmembrane protein with an extracellular (a.a. 24-182) composed of three TNFR-Cys repeats (a.a. 26-63, 64-104, 105-141), followed by a transmembrane region (a.a. 183-203), and a cytoplasmic tail (a.a. 204-250).

Specifications

• Antigen: CD27
• Classification: Monoclonal
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified rat IgG2a in PBS without preservatives.
• Gene Accession No.: P41272
• Immunogen: Human IgG1 Fc-tagged recombinant mouse CD27 extracellular domain (Sumi, T., et al. (2008). Immunol. Lett. 119(1-2):91-96).
• Quantity: 100 μL
• Research Discipline: Inflammation & Immunology
• Gene ID (Entrez): NP_001028298
• Target Species: Mouse
• Form: Purified

• Applications: Neutralization
• Clone: RM27-3E5
• Conjugate: Unconjugated
• Gene: Cd27, Tnfrsf7
• Host Species: Rat
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone RM27-3E5 enhanced anti-CD3-stimulated T-cell proliferation when immobilized in vitro and augmented OVA-specific CTL induction when administrated in vivo (Roberts, D.J., et al. (2010). J. Immunother. 33(8): 769 779; Sumi, T., et al. (2008). Immunol. Lett. 119(1-2):91-96).
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Isotype: IgG2a κ

For Research Use Only. Not for use in diagnostic procedures.