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CD273 (B7-DC) Rat anti-Mouse, PE, Clone: 122, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

Manufacturer:  Invitrogen 12997281

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Catalog No. 50-113-32



Description

Description

Description: The 122 monoclonal antibody reacts with mouse B7-DC, also known as PD-L2. B7-DC, a recently identified member of the B7 family, has a predicted molecular weight of approximately 25 kDa and belongs to the Ig superfamily. The mouse B7-DC has a short cytoplasmic tail (4aa). B7-DC is primarily expressed by subpopulations of dendritic cells and monocytes/macrophages in the mouse. Although B7-DC has structural and sequence similarities to the B7 family, it does not bind CD28/CTLA-4, rather it is a ligand for PD-1. The interactions between PD-1 and B7-DC/PD-L2 have been reported to be involved in costimulation or suppression of T cell proliferation depending on state of cellular activation. 122 has been demonstrated to block binding of TY25 (Cat. No.14-5986), another mAb specific for mouse B7-DC. Applications Reported: 122 has been reported for use in flow cytometric analysis. Applications Tested: The 122 antibody has been tested by flow cytometric analysis of mouse dendritic cells or mouse B7-DC transfected cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered. Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by co gnate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PDL-2 can act to stimulate an immunogenic response through and alternative receptor from PD-1. At least three isoforms of PDL-2 are known to exist; this antibody is specific to the largest isoform. PDL-2 antibody has no cross-reactivity to PDL-2.
Specifications

Specifications

CD273 (B7-DC)
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q9WUL5
B7-DC, Btdc, F730015O22Rik, MGC124039, MGC124040, PD-L2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
122
PE
Pdcd1lg2
Liquid
Pdcd1lg2
IgG2a, kappa
50 μg
4° C, store in dark, DO NOT FREEZE!
Primary
58205
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