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CD274, NA/LE Rat anti-Mouse, Unlabeled, Clone: MIH5, BD

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 565772

Catalog No. BDB565772


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Description

Description

The MIH5 monoclonal antibody specifically binds to CD274, also known as B7-H1 or PDL1, a 43-kDa glycoprotein encoded by the Pdcd1lg1 /gene of the B7 family of the Ig superfamily. Pdcd1lg1 /mRNA is expressed in more tissues than other members of the B7 family; transcripts are found in lymphoid tissues and many, but not all, non-lymphoid tissues. The protein has been detected at low levels on resting peripheral T and B lymphocytes, macrophages, and dendritic cells. B7-H1 mRNA and protein expression are upregulated upon activation of T and B cells, macrophages, dendritic cells, and epidermal keratinocytes by a variety of stimulatory factors. B7-H1's receptor, PD-1, contains an ITIM ( I /mmunoreceptor T /yrosine-based I /nhibitory M /otif) on its intracytoplasmic region and is expressed on activated B and T lymphocytes, suggesting that B7-H1-PD-1 interaction may be involved in the negative regulation of immune responses. The second PD-1 ligand, B7-DC (PD-L2), is also a member of the B7 family of the Ig superfamily. Furthermore, B7-H1 may participate in positive immunoregulation, or costimulation of T cells, through an additional receptor, which is not PD-1 and distinct from the alternate receptor for B7-DC. The MIH5 antibody blocks the binding of PD-1-Ig to B7-H1 transfectants.

NA/LE
Specifications

Specifications

CD274, NA/LE
1.0mg/mL
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered. Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
B7-H1, PD-L1, PD1L1, Programmed death ligand 1
DBA/2 mouse lymphoma L5178Y transfected with Pdcd1lg1 cDNA
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
RUO
Primary
Murine
MIH5
Unconjugated
Affinity Purified
Rat
Rat SD, also known as Sprague-Dawley (outbred) IgG2a, λ
500μg
Store undiluted at 4°C.
Monoclonal
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