CD275 (B7-H2) Mouse anti-Human, PerCP-eFluor 710, Clone: MIH12, eBioscience
Mouse Monoclonal Antibody
Manufacturer: eBioscience 46588941
Description: The MIH12 monoclonal antibody reacts with human B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. Human B7RP-1 is expressed by activated monocytes/macrophages. B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells. The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway. Applications Reported: This MIH12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This MIH12 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood monocyte-derived dendritic cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS). ICOS belongs to the CD28/CD152 receptor family that regulates T-cell activation and function. ICOSL is expressed on monocytes, dendritic cells and B cells and can be induced by inflammatory stimuli in peripheral tissue. Binding to ICOSL delivers a co-stimulatory signal for T cell proliferation and cytokine secretion.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|4° C, store in dark, DO NOT FREEZE!|
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