CD279 (PD-1) Monoclonal Antibody (J43), Functional Grade, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-148-22	Invitrogen 16998582	100 μg
50-148-23	Invitrogen 16998585	500 μg

Description

Description: The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2. Applications Reported: The J43 antibody has been reported for use in flow cytometric analysis. It has also been reported for use in in vitro functional assays. Applications Tested: The J43 antibody has been tested by flow cytometric analysis of mouse Con-A activated spleen cells and mouse PD-1 transfected cells. This can be used at less than or equal to 0.5 μg per test.

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "e;danger signals"e; such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

Specifications

• Antigen: CD279 (PD-1)
• Concentration: 1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with no preservative; pH 7.2
• Gene Alias: CD279; EGK_05005; hPD1; hPD-1; hPD-l; hSLE1; Ly101; mPD-1; PD1; PD-1; Pdc1; Pdcd1; programmed cell death 1; programmed cell death 1 protein; programmed cell death protein 1; programmed cell death protein 1-like; programmed death 1; Protein PD1; protein PD-1; sCD279; SLEB2; soluble CD279; systemic lupus erythematosus susceptibility 2
• Gene Symbols: Pdcd1
• Primary or Secondary: Primary
• Content And Storage: 4° C
• Gene: Pdcd1

• Clone: J43
• Applications: Flow Cytometry, Functional Assay
• Conjugate: Functional Grade
• Host Species: Armenian Hamster
• Target Species: Mouse
• Gene Accession No.: Q02242
• Gene ID (Entrez): 18566
• Isotype: IgG
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.