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CD28 Syrian Hamster anti-Mouse, PerCP-Cyanine5.5, Clone: 37.51, eBioscience™ Non-distribution product as customer accommodation.

Syrian Hamster Monoclonal Antibody

Manufacturer:  Invitrogen 45028180

Catalog No. 50-157-86


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Description

Description

Description: The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells. Applications Reported: This 37.51 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 37.51 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary’s Disease.
Specifications

Specifications

CD28
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P31041
MGC138290, Tp44, CD28 antigen, T-cell-specific surface glycoprotein CD28
Syrian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
37.51
PerCP-Cyanine5.5
Cd28
Liquid
Cd28
IgG
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
12487
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