CD294 (CRTH2) Rat anti-Mouse, Alexa Fluor® 647, Clone: No3m1scz, eBioscience
Rat Monoclonal Antibody
Manufacturer: eBioscience 51294182
Description: This clone recognizes mouse CD294, also known as Prostaglandin D2 receptor 2 (PD2R2), G-protein coupled receptor 44 (GPR44) or Chemoattractant Receptor-Homologous Molecule Expressed On TH2 Cells (CRTH2). Although the clone works on unfixed mouse CD294-transfected cells, we recommend fixing and permeabilizing primary cells. Applications Reported: This No3m1scz antibody has been reported for use in flow cytometric analysis. Applications Tested: This No3m1scz antibody has been tested by flow cytometric analysis of mouse lysed whole blood cells using Protocol A: Two-step protocol for intracellular (cytoplasmic) proteins. This allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Use of Protocol B: One-step protocol for intracellular (nuclear) proteins is recommended for staining of transcription factors in conjunction with surface and phosphorylated intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to “Clone Performance Following Fixation/Permeabilization” located in the Best Protocols Section under the Resources tab online). All Protocols can be found in the “Staining Intracellular Antigens for Flow Cytometry Protocol” located in the Best Protocols Section under the Resources tab online.This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser The prostaglandin D2 receptor 2 (PTGDR2), also known as CRTH2, is expressed on Th2 cells and eosinophils and mediates chemotaxis of these cells to PGD2 and is thus thought to be a key receptor mediating eosinophil and Th2 recruitment during allergic responses. However, PTGDR2-null mice showed enhanced eosinophil recruitment into the lung consistent with observations that the PTGDR2-null mice produced significantly higher amounts of interleukin-5 (IL-5) and IL-3. This suggests that PTGDR2 plays a nonredundant role in restricting eosinophilia and allergic response in vivo.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|CRTH2, DP2, PD2R2, GPR44, DL1R|
|4° C, store in dark, DO NOT FREEZE!|
|Alexa Fluor™ 647|
|rat cell line transfected with mouse CD294|
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