CD3 delta Mouse anti-Rat, Clone: 1F4, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NB10065230

Catalog No. NB10065230

Description

CD3 delta Monoclonal specifically detects CD3 delta in Rat samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

• Antigen: CD3 delta
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Flow Cytometry 1:10-1:25, Immunohistochemistry 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10, Immunohistochemistry-Frozen 1:10-1:25
• Gene Symbols: CD3D
• Immunogen: F344 rat T cells stimulated with PMA (TPA) and calcium ionophore
• Quantity: 0.1 mg
• Research Discipline: Adaptive Immunity, Apoptosis, Diabetes Research, Immunology, Innate Immunity, Signal Transduction
• Gene ID (Entrez): 915
• Target Species: Rat
• Form: Purified

• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: 1F4
• Conjugate: Unconjugated
• Gene Alias: CD3 antigen, delta subunit, CD3d antigen, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule, delta (CD3-TCR complex), CD3-DELTA, T3DOKT3, delta chain, T-cell receptor T3 delta chain, T-cell surface glycoprotein CD3 delta chain
• Host Species: Mouse
• Purification Method: IgM purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes Rat CD3D (Gene ID: 25710, UniProt: P19377) and Rat CD3G (Gene ID: 300678, UniProt: Q64159) NB100-65230 recognizes rat CD3, a 25kD antigen which is found on rat T-cells. NB100-65230 does not react with rat B cells. In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex. Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgM

For Research Use Only