CD3 Mouse anti-Human, Alexa Fluor 647, Clone: MEM-57, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 566334
The human CD3 complex consists of four transmembrane proteins (γ, δ, ε, ζ) that are associated withthe T cell antigen receptor (TCR) to form the CD3/TCR complex. The CD3 complex associates witheither TCR αβ or TCR γδ heterodimersthat are expressed by some thymocytes, T cells or NKT cells. The CD3 complex is required for the cell surface expression and signal-transducing functions of the TCR. The CD3 complex is expressed by ñ60-85% thymocytes and ñ60-80% of normal human peripheral blood lymphocytes. The MEM-57 monoclonal antibody reportedly recognizesthe human CD3 epsilon subunit (CD3e/CD3ε, also known as T3E or TCRE) of the CD3/TCR complex. CD3e is a ñ20 kDa unglycosylated transmembrane protein that belongs to the Ig gene superfamily. CD3e has an Ig-like extracellular domain (ECD) and an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The MEM-57 antibody reportedly stimulates T cell proliferation and can induce cytotoxicity in some human T cell lines.Alexa Fluor™ 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor™ 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor™ 647 cannot be used simultaneously.
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|CD3-epsilon, CD3E, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, TCRE|
|Human Thymocytes and T Lymphocytes|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor™ 647 under optimum conditions, and unreacted Alexa Fluor™ 647 was removed.|
|Alexa Fluor 647|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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