CD30 Mouse anti-Human, BUV395, Clone: BerH8 (also known as Ber-H8), BD Optibuild™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 744412

Catalog No. BDB744412

Description

The BerH8 monoclonal antibody specifically binds to CD30, a 120 kDa type I transmembrane glycoprotein expressed on stimulated T and B cells. CD30 is an activation marker initially identified to be expressed on Reed-Sternberg cells. It is also expressed on a few extrafollicular T and B cells located at the rim of germinal centers. CD30 serves as a cytokine receptor. It belongs to the nerve growth factor receptor/tumor necrosis factor receptor (NGFR/TNFR) superfamily and is also known as TNFRSF8. CD30 interaction with CD30 ligand (CD30L/CD153/TNFSF8) can mediate signals for proliferation, apoptosis and cytotoxicity of lymphoid cells.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter. BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.

Specifications

• Antigen: CD30
• Clone: BerH8 (also known as Ber-H8)
• Conjugate: Brilliant Ultraviolet 395
• Gene Alias: TNFRSF8, TNR8, CD30L/CD153 receptor, Ki-1, D1S166E
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG1 κ

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.