Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

CD307d (FcRL4), PerCP-eFluor™ 710, clone: 413D12, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 46307941

 View more versions of this product

Catalog No. 50-113-0799


Add to cart

Description

Description

This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein has four extracellular C2-type immunoglobulin domains, a transmembrane domain and a cytoplasmic domain that contains three immune-receptor tyrosine-based inhibitory motifs. This protein may play a role in the function of memory B-cells in the epithelia. Aberrations in the chromosomal region encoding this gene are associated with non-Hodgkin lymphoma and multiple myeloma.

Description: The 413D12 monoclonal antibody reacts with human CD307d, which is also known as FcRL4, Fc receptor-like 4, FcRH4, IRTA4, or IGFP2. CD307d is an inhibitory receptor that is highly expressed on a subset of memory B cells resident in epithelial tissues and marginal zones of mucosal lymphoid tissues such as tonsils. CD307d has been shown to recruit the phosphatase SHP-1, thereby acting as an inhibitory receptor in B cells. CD307d is homologous to FcgammaRI and specifically binds IgA. To date, CD307d is the only known inhibitory receptor for IgA. Expression of CD307d is increased on memory B cells from patients with chronic infections such as HIV, suggesting that enhanced expression of inhibitory receptors, such as CD307d, may contribute to the inefficiency of antibody responses against HIV.Applications Reported: This 413D12 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 413D12 antibody has been pre-titrated and tested by flow cytometric analysis of human tonsil tissue or transfected 293T cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2µm post-manufacturing filtered.
Specifications

Specifications

CD307d (FcRL4)
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q96PJ5
FCRH4, IGFP2, IRTA1, MGC150522, MGC150523
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
413D12
PerCP-eFluor 710
FCRL4
Liquid
FCRL4
IgG2b, kappa, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
83417
Documents
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only.