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CD314 (NKG2D) Mouse anti-Human, Clone: 1D11, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

$215.00

Specifications

Antigen CD314 (NKG2D)
Clone 1D11
Target Species Human
Conjugate Unconjugated
Isotype IgG1, kappa
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Products 1
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-132-55 Non-distribution product as customer accommodation.
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Invitrogen
14587882
100 μg
Each for $215.00
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Description

Description

Description: The 1D11 monoclonal antibody reacts with the human NKG2D, a 42 kDa lectin-like molecule expressed by NK cells, gamma delta T cells, CD8+ T cells, and some CD4+ T cells. Human NKG2D forms complexes with DAP10, a membrane adaptor protein, and has the ability to costimulate multiple NK activation receptors. The counter-receptor for human NKG2D has been identified as MICA/MICB expressed on epithelial tumors from lung, breast, kidney, ovary, prostate and colon carcinoma. 5C6 and 1D11 block binding of soluble MICA to gamma delta TCR T cell clones and inhibit lysis by these cells. 5C6 and 1D11 induced NKG2D function of redirected lysis of FcReceptor bearing P815 cells. Applications Reported: The 1D11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. It has also been reported in inhibition of ligand binding in in vitro assays. Please use Functional Grade Purified 1D11 (cat. 16-5878) in functional assays. (Fluorochrome-conjugated 1D11 is recommended for use in flow cytometric analysis.). Applications Tested: The 1D11 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL.

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. This gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.
Specifications

Specifications

CD314 (NKG2D)
Human
IgG1, kappa
Monoclonal
Liquid
Antibody
4° C
1D11
Unconjugated
Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation, Neutralization
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.

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