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CD317 (BST2, PDCA-1) Rat anti-Mouse, Functional Grade, Clone: eBio927, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

$119.00 - $375.00

Specifications

Antigen CD317 (BST2, PDCA-1)
Clone eBio927
Target Species Mouse
Conjugate Functional Grade
Isotype IgG2b, kappa
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-145-15 Non-distribution product as customer accommodation.
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Invitrogen
16317281
50 μg Each for $119.00
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50-145-17 Non-distribution product as customer accommodation.
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Invitrogen
16317285
500 μg Each for $375.00
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Description

Description

Description: The eBio927 monoclonal antibody reacts with PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naive mouse. Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predomitly expressed by IPCs in the naive mouse which represents a very minor population (<0.5%) of splenocytes. It is upregulated on numerous cell types following stimulation which triggers an IFN response. PDCA-1 cycles between cell surface and intracellular compartments and may function to regulate trafficking of secreted cytokines. PDCA-1 (BST2) is the protein recognized by antibody 120G8. The eBio927 monoclonal antibody has also been shown to have functional activity. The epitope recognized by eBio927 is distinct from eBio129c; thus, the antibodies can be used to costain, purify and identify pDCs. Applications Reported: This eBio927 antibody has been reported for use in flow cytometric analysis and for in vivo depletion studies as well as in vitro crosslinking studies. Applications Tested: This eBio927 antibody has been tested by flow cytometric analysis of BST-2 transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC. BST2, also known as CD317, HM1.24 antigen, DAMP-2 and recently designated tetherin, is an integral membrane protein with a novel topology. The specific function of the BST2, encoded by the bone marrow stromal cell antigen 2 gene, is undetermined; however, it may play a role in pre-B-cell growth, rheumatoid arthritis, and in antiretroviral defense, that blocks release of retrovirus from the cell surface. BST2 is highly expressed during B-cell development, from pro-B precursors to plasma cells, T-cells, monocytes, NK cells and DCs (at protein level).
Specifications

Specifications

CD317 (BST2, PDCA-1)
Mouse
IgG2b, kappa
Monoclonal
Liquid
Antibody
4° C
eBio927
Functional Grade
Flow Cytometry, Functional Assay
Primary
Monoclonal
PBS with no preservative; pH 7.2
RUO

For Research Use Only.

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