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CD335 (NKp46) Rat anti-Mouse, APC-eFluor® 780, Clone: 29A1.4, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

$142.00 - $328.00

Specifications

Antigen CD335 (NKp46)
Clone 29A1.4
Target Species Mouse
Conjugate APC-eFluor™ 780
Isotype IgG2a, kappa
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-112-9221 Non-distribution product as customer accommodation. View Documents Invitrogen
47335180
25 μg Each for $142.00
Add to cart
 
50-112-4054 Non-distribution product as customer accommodation. View Documents Invitrogen
47335182
100 μg Each for $328.00
Add to cart
 
Description

Description

Description: The monoclonal antibody 29A1.4 recognizes mouse NKp46, also known as CD335. CD335, a member of the natural cytotoxicity receptor (NCR) family, is a glycoprotein with 2 Ig-like domains and a short cytoplasmic tail. Expression of CD335 is uniquely found on NK cells (including immature NK cells, defined as DX5- CD3-, and thereby allowing discrimination between NKT cells and NK cells (NKp46+, CD3-). Furthermore, unlike many of the NK markers which also stain NKT cells, staining with 29A1.4 is not strain specific. Staining has been shown on C57Bl/6, SJL, CBA/CA and BALB/C strains. NKp46 has been shown to play a role in NK cell-mediated lysis of several tumor cells and pathogen-infected cell lines. The 29A1.4 monoclonal antibody has been shown to activate NK cells in vitro. The 29A1.4 monoclonal antibody does not deplete NK cells in vivo. Applications Reported: This 29A1.4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 29A1.4 antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered. Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
Specifications

Specifications

CD335 (NKp46)
Mouse
IgG2a, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
29A1.4
APC-eFluor™ 780
Flow Cytometry
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.

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