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CD336 (NKp44) Mouse anti-Human, PerCP-eFluor™ 710, Clone: 44.189, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 46336942

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Catalog No. 50-112-3806


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Description

Description

Description: The monoclonal antibody 44.189 reacts with human CD336, which is also known as NKp44 or NCR2. CD336 is a 44-kDa transmembrane glycoprotein belonging to the natural cytotoxicity receptor (NCR) family and contains an ITIM domain located in the cytoplasmic tail. Expression of CD336 and NK cell activation by the receptor requires association with DAP12, an ITAM-containing transmembrane accessory protein. Once thought to be expressed exclusively on IL-2-activated NK cells and a subset of gamma delta T lymphocytes, studies have shown expression of CD336 on IL-3-stimulated plasmacytoid dendritic cells, alpha beta T cells in the small intestinal epithelium, and T cells in cord blood. CD336 has been shown to be involved in the innate response of activated NK cells against viral infection and tumor cell proliferation. The 44.189 antibody has been reported to facilitate CD336 crosslinking. Applications Reported: This 44.189 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 44.189 antibody has been pre-titrated and tested by flow cytometric analysis of IL-2-stimulated normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered. CD336 is also known as NKp44 or NCR2. CD336 is a 44-kDa transmembrane glycoprotein belonging to the natural cytotoxicity receptor (NCR) family and contains an ITIM domain located in the cytoplasmic tail. Expression of CD336 and NK cell activation by the receptor requires association with DAP12, an ITAM-containing transmembrane accessory protein. Once thought to be expressed exclusively on IL-2-activated NK cells and a subset of gamma delta T lymphocytes, studies have shown expression of CD336 on IL-3-stimulated plasmacytoid dendritic cells, alpha beta T cells in the small intestinal epithelium, and T cells in cord blood. CD336 has been shown to be involved in the innate response of activated NK cells against viral infection and tumor cell proliferation.
Specifications

Specifications

CD336 (NKp44)
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
O95944
CD336, LY95, NCR2, CD336, CD336 antigen, LY95lymphocyte antigen 95 homolog (activating NK-receptor, NK-p44), lymphocyte antigen 95 (activating NK-receptor, NK-p44), Lymphocyte antigen 95 homolog, natural cytotoxicity triggering receptor 2, Natural killer cell p44-related protein, NK cell activating receptor (NKp44), NK cell-activating receptor, NKp44, NKP44, NK-p44dJ149M18.1
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
44.189
PerCP-eFluor™ 710
NCR2
Liquid
NCR2
IgG2b, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
9436
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