CD36 Mouse anti-Human, PerCP-eFluor 710, Clone: eBioNL07 (NL07), eBioscience
Mouse Monoclonal Antibody
Manufacturer: Invitrogen 46036941
Description: The monoclonal antibody eBioNL07 recognizes human CD36, which is a member of the class B scavenger receptor family. CD36 was originally identified as a platelet-membrane glycoprotein also called glycoprotein IV and a receptor for thrombospondin-1 (TSP-1) and extracellular matrix proteins. Binding to TSP-1 is in the CLESH (CD36 LIMP-II Emp sequence homology) domain of CD36. CD36 expression is broad and includes microvascular (but not large vessel) endothelium, adipocytes, skeletal muscle, dendritic cells, epithelia of the retina, breast, and intestine, smooth muscle cells, and hematopoietic cells, including erythroid precursors, platelets, monocytes/macrophages, DCs and megakaryocytes. Expression on platelets is absent on Nak-a negative donors. Unlike other scavenger receptor, CD36 binds LDL that has been exposed to "minimally" oxidizing conditions. CD36 is also a fatty acid translocase (FAT) necessary for the transport of long-chain fatty acids (LCFAs) and therefore may play a role in atherosclerosis. Applications Reported: This eBioNL07 (NL07) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioNL07 (NL07) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral bloocd cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL.Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered. CD36 (fatty acid translocase, FAT) is an 88 kDa, ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and is rapidly induced on Follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similar to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids. CD36 is preferentially found within lipid rafts, which facilitates its association with receptors, signaling and adapter molecules. CD36 binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. Further, CD36 may function as a cell adhesion molecule and directly mediates cyto-adherence of Plasmodium falciparum parasitized erythrocytes. Mutations in the CD36 gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants encoding the CD36 protein have been found.
|PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2|
|FAT, GPIV, Scarb3, GPIIIB, PAS IV, PAS-4, fatty acid translocase, glycoprotein IIIb, platelet glycoprotein 4, platelet glycoprotein IV|
|4° C, store in dark, DO NOT FREEZE!|
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