CD365 (TIM-1) Rat anti-Mouse, Brilliant Violet 421, Clone: RMT1-4, BD
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 566336
The RMT1-4 monoclonal antibody specifically recognizes CD365 which is encoded by Havcr1 /(Hepatitis A virus cellular receptor 1) and commonly known as T cell immunoglobulin and mucin domain-containing protein 1 (TIM-1). TIM-1 is a type I transmembrane glycoprotein that contains an extracellular immunoglobulin V-like domain and mucin-like domain and a conserved intracellular tyrosine phosphorylation motif that is involved in transmembrane signaling. TIM-1 is a costimulatory molecule that plays an important role in regulating immune responses. It is expressed on mast cells, and activated T cells including T helper type 2 (Th2) cells. TIM-1 is constitutively expressed by some B cells with increased TIM-1 expression detected upon B cell activation. TIM-1 was reportedly expressed by the majority of IL-10-expressing regulatory B cells within several major splenic B cell subsets. These cells may play critical roles in Th2-like CD4 T cell differentiation and in the regulation of inflammation and autoimmunity. TIM-4, a phosphatidylserine receptor encoded by Timd4 /, is a putative ligand for TIM-1.The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
|Havcr1, Tim1, TIMD-1, Timd1, KIM-1, Kidney injury molecule 1|
|Mouse TIM-1 Recombinant Protein|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV421 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV421 were|
|Brilliant Violet 421|
|Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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