CD366 (TIM3) Monoclonal Antibody (F38-2E2), Super Bright™ 436, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 62310942

Catalog No. 62-310-942

Description

Description: This F38-2E2 monoclonal antibody reacts with human CD366, also known as T cell immunoglobulin and mucin domain-containing protein 3 (TIM3) or HAVCR2.  This cell surface receptor is expressed on activated CD4+ T cell subsets (e.g. Th1, Th17, and Treg), CD8+ T cells, monocytes, dendritic cells, and mast cells.  Due to alternative splicing, CD366 exists as membrane-bound and soluble forms.  Galectin-9 has been identified as the ligand for CD366.  In humans, this receptor negatively regulates CD4+  T cells, influencing the secretion of some Th1- and Th17-related cytokines.  CD366 has also been implicated in tolerance, autoimmune disease (e.g. multiple sclerosis), and HIV infection. Applications Reported: This F38-2E2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This F38-2E2 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

CD366 (TIM3) Monoclonal antibody specifically detects CD366 (TIM3) in Human samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD366 (TIM3)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q8TDQ0
• Gene Symbols: Havcr2
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 84868
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: F38-2E2
• Conjugate: Super Bright 436
• Gene: Havcr2
• Gene Alias: CD366; FLJ14428; Havcr2; HAVcr-2; Hepatitis A virus cellular receptor 2; hepatitis A virus cellular receptor 2 homolog; kidney injury molecule-3; KIM-3; sCD366; soluble CD366; soluble TIM 3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain containing 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin family member 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; Tim3; TIM-3; TIMD3; TIMD-3
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.