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CD366 (TIM3) Mouse anti-Human, Super Bright 702, Clone: F38-2E2, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 67310942

Encompass

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Catalog No. 67-310-942


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Description

Description

Description: The rat IgG2b monoclonal antibody is useful as an isotype control immunoglobulin. Applications Reported: Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis. Applications Tested: Rat IgG2b Isotype Control has been tested by flow cytometric analysis of mouse splenocytes. This can be used at the same concentration as the experimental antibody. Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No. SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 702 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2, T-cell immunoglobulin, mucin-dmain containing-3) is a 281 amino acid long, Type-1 Th1- specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. TIM3 consists of an IgV-like domain, a mucin-like domain in the extracellular region, and a conserved Tyrosine phosphorylation motif in the cytoplasmic region. TIM3 is involved in macrophage activation and induction of autoimmune diseases. Further, TIM3 down-regulates aggressive Th1-mediated immune responses and facilitates in the development of immune tolerance. Pathological significance of TIM3 has been attributed to Experimental autoimmune encephalomyelitis (EAE), a Th-1 dependent autoimmune disease, and also enhances the severity of experimental autoimmune encephalomyelitis in mice.
Specifications

Specifications

CD366 (TIM3)
Monoclonal
5 μL/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
Q8TDQ0
CD366; HAVcr-2; HAVCR2; KIM-3; Tim-3; TIM3; TIMD-3; TIMD3
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
F38-2E2
Super Bright 702
HAVCR2
Liquid
HAVCR2
IgG1, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
84868
SDS
Documents
Product Certifications
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