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CD369 (Clec7a, Dectin-1) Mouse anti-Human, Non-human primate, eFluor® 660, Clone: 15E2, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 50985642

Catalog No. 50-112-4561


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Description

Description

Description: This 15E2 monoclonal antibody reacts with human and non-human primate CD369, which is also known as the Dectin-1 or beta-glucan receptor or CLEC7A. CD369 is expressed predomitly on monocytes, macrophages, and dendritic cells. Moreover, this receptor can also be detected on neutrophils and lymphocytes, as well as induced by cytokines such as IL-4 or LPS. In humans, CD369 exists as two isoforms that arise due to alternative splicing. CD369 binds beta-1,3-glucan and plays an important role in activating signal transduction involved in the innate immune response to fungal and mycobacterial infections. Studies have also shown the involvement of CD369 in dendritic cell activation. The 15E2 monoclonal antibody has been reported to recognize both isoforms of CD369 and activate dendritic cells. It does not crossreact to mouse CD369. Applications Reported: Anti- Dectin-1 eFluor™ 660 has been reported for use in flow cytometric analysis. Applications Tested: This 15E2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µL) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor™ 660 is a replacement for Alexa Fluor™ 647.

eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered. CLEC7A, also known as dectin-1, is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily and is predomitly expressed on myeloid cells. It is a small glycoprotein type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif (ITAM). CLEC7A functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, triggering a massive oxidative burst through the formation of reactive oxygen species.
Specifications

Specifications

CD369 (Clec7A, Dectin-1)
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q9BXN2
UNQ539/PRO1082, BGR, CANDF4, CLECSF12, DECTIN1,C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 12; C-type lectin domain family 7 member A; C-type lectin superfamily member 12; DC-associated C-type lectin 1; beta-glucan receptor; dectin-1; dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; lectin-like receptor 1
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Non-human Primate
Flow Cytometry
15E2
eFluor™ 660
CLEC7A
Liquid
CLEC7A
IgG2a, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
64581
Documents
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