CD3e Monoclonal Antibody (145-2C11), NovaFluor™ Blue 660-120S, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Supplier:  LIFE TECHNOLOGIES M002T02B08

Catalog No. M002T02B08

Description

The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.
Applications Reported: This 145-2c11 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 145-2c11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 1.0 μg per test. A test is defined as the amount ( μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
When using NovaFluor dyes, CellBlox Moncyte and Macrophage Blocking Buffer is recommended to block non-specific binding of dyes to monocytes.

Excitation: 496 nm; Emission: 511 nm; Laser: 488 nm (Blue) Laser

CD3e Monoclonal antibody specifically detects CD3e in Mouse samples. It is validated for Flow Cytometry

Specifications

• Antigen: CD3e
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: P04235, P11942, P22646, P24161
• Host Species: Armenian Hamster
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: Flow Cytometry
• Clone: 145-2C11
• Conjugate: NovaFluor Blue 660-120S
• Gene: Cd247
• Gene Alias: 4930549J05Rik; A430104F18Rik; AI504783; antigen CD3D, delta polypeptide (TiT3 complex); antigen CD3E, epsilon polypeptide (TiT3 complex); antigen CD3G, gamma polypeptide; antigen CD3Z, zeta polypeptide; AW552088; CD247; CD247 antigen; CD247 antigen, zeta subunit; CD247 molecule; Cd3; CD3 antigen delta chain; CD3 antigen delta polypeptide; CD3 antigen gamma chain; CD3 antigen, delta polypeptide; CD3 antigen, delta subunit; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 delta; CD3 epsilon; CD3 epsilon chain; CD3 epsilon subunit; CD3 epsilon subunit precursor; CD3 gamma-chain; CD3 glycoprotein; CD3 molecule delta polypeptide; CD3 molecule, delta; CD3 molecule, epsilon; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma; CD3 molecule, gamma polypeptide; CD3 protein; CD3 type I transmembrane glycoprotein; CD3 zeta chain; Cd3d; CD3D antigen delta; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule; CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e; CD3E antigen, epsilon polypeptide; CD3E antigen, epsilon polypeptide (TiT3 complex); CD3e molecule; CD3e molecule, epsilon (CD3-TCR complex); CD3epsilon; CD3-epsilon; Cd3-eta; CD3g; CD3G antigen, gamma polypeptide; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule; CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3-GAMMA; CD3H; CD3Q; CD3Z; CD3Z antigen, zeta polypeptide (TiT3 complex); Cd3zeta; CD3-ZETA; CD3zeta chain; CD3-zeta/eta; Ctg3; Ctg-3; FLJ18683; IMD17; IMD18; IMD19; IMD25; Leu-4; OKT3, delta chain; T3/TCR complex; T3D; T3E; T3G; T3z; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor CD3 epsilon chain; T-cell receptor CD3 epsilon subunit; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell receptor zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T-cell surface protein; TcR CD3 delta-chain; TcR CD3 gamma-chain; TCR zeta chain; TCR zeta chain subunit; TCRE; Tcrk; TCRZ; TCRzeta; TiT3 complex
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12500, 12501, 12502, 12503
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid