CD4 Mouse anti-Human, PerCP-Cy5.5, Clone: SK3 (also known as Leu3a), BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 566316

Catalog No. BDB566316

Description

The SK3 monoclonal antibody specifically binds to CD4. The CD4 antigen is a 55 kDa type 1 transmembrane glycoprotein that belongs to the immunoglobulin superfamily. It is expressed on T-helper/inducer lymphocytes and monocytes. CD3+CD4+ T cells comprise approximately 28% to 58% of normal peripheral blood lymphocytes. CD4 is also expressed on 80% to 95% of normal thymocytes. The CD4 antigen is present in low density on the cell surface of monocytes and in the cytoplasm of monocytes and macrophages (CD3-CD4+). CD4 serves as a T cell coreceptor for MHC class II antigen recognition and as a receptor for the human immunodeficiency virus (HIV). The SK3 antibody inhibits HIV binding to CD4+ cells.

PerCP-Cy™5.5 is a tandem conjugate that combines PerCP with a cyanine dye. PerCP-Cy5.5 is not subject to photobeaching like PerCP and can be used with stream-in-air flow cytometers. It has less Fc receptor–mediated nonspecific staining than PE-Cy5. Additionally, the PerCP-Cy5.5 tandem conjugate is not as susceptible to fixative or light instability compared to APC-Cy7 and PE-Cy7.

Specifications

• Antigen: CD4
• Conjugate: PerCP-Cyanine5.5
• Format: Affinity Purified
• Host Species: Mouse
• Isotype: Mouse BALB/c IgG₁, κ
• Quantity: 25 Tests
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal

• Clone: SK3 (also known as Leu3a)
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Gene Alias: L3T4, Leu3a, T-cell surface antigen T4/Leu-3, W3/25, CD4 antigen (p55)
• Immunogen: Human Peripheral Blood T Cells
• Purification Method: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerC
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human