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CD4 Rat anti-Mouse, Clone: 4SM95, eBioscience™ Invitrogen™

Rat Monoclonal Antibody

Manufacturer:  LIFE TECHNOLOGIES 14976695

Encompass

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Catalog No. 14-976-695


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Description

Description

This 4SM95 MAb reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T cells, and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. The 4SM95 MAb is not recommended for immunohistochemistry of frozen mouse tissue. For detection of mouse CD4 using immunohistochemistry of frozen tissue please refer to alternate products Anti-Mouse CD4 Purified (cat. 14-0041 and 14-0042). Applications Reported: This 4SM95 antibody has been reported for use in western blotting, microscopy, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This 4SM95 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. This 4SM95 antibody has also been tested by western blot of reduced mouse spleen lysate and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

CD4 Monoclonal antibody specifically detects CD4 in Mouse samples. It is validated for Immunohistochemistry (Paraffin), Western Blot
Specifications

Specifications

CD4
Immunohistochemistry (Paraffin), Western Blot
4SM95
Unconjugated
CD4
CD4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; Cd4 molecule; CD4 receptor; CD4, allele 1; cd4a; CD4mut; cell surface glycoprotein CD4; fCD4; L3T4; Leu-3; Ly-4; lymphocyte antigen CD4; lymphocyte antigen CD4 precursor; p55; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); W3/25; W3/25 antigen
IgG1, kappa
10 mg
4° C
Primary
12504
Liquid
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P06332
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
SDS
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Product Certifications
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