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CD4 Rat anti-Mouse, NovaFluor™ Blue 660-120S, Clone: GK1.5, Invitrogen™

Rat Monoclonal Antibody

Manufacturer:  LIFE TECHNOLOGIES M001T02B08

Encompass

Catalog No. M001T02B08


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Description

Description

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.
Applications Reported: This GK1.5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This GK1.5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.2 μg per test. A test is defined as the amount ( μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
When using more than one NovaFluor dye, CellBlox Monocyte and Macrophage Blocking Buffer may be used to block non-specific binding of dyes to monocytes.
Excitation: 509 nm; Emission: 665 nm; Laser: 488 nm (Blue) Laser

CD4 Monoclonal antibody specifically detects CD4 in Mouse samples. It is validated for Flow Cytometry
Specifications

Specifications

CD4
Monoclonal
0.1 mg/mL
PBS with no preservative; pH 7.2
P06332
CD4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; Cd4 molecule; CD4 receptor; CD4, allele 1; cd4a; CD4mut; cell surface glycoprotein CD4; fCD4; L3T4; Leu-3; Ly-4; lymphocyte antigen CD4; lymphocyte antigen CD4 precursor; p55; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); W3/25; W3/25 antigen
IgG2b, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
12504
Flow Cytometry
GK1.5
NovaFluor Blue 660-120S
CD4
Liquid
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
SDS
Documents
Product Certifications
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