CD4 Monoclonal Antibody (RM4-4), PE-eFluor™ 610, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
61-004-382	eBioscience 61004382	100 μg

Description

Description: The RM4-4 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by the majority of thymocytes, a subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with the protein tyrosine kinase lck through its cytoplasmic tail. Binding of RM4-4 does not block binding of the CD4 monoclonal antibodies RM4-5 or GK1.5. Applications Reported: This RM4-4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This RM4-4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red).  Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation.

CD4 Monoclonal antibody specifically detects CD4 in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD4
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P06332
• Isotype: IgG2b κ
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

• Clone: RM4-4
• Applications: Flow Cytometry
• Conjugate: PE-eFluor 610
• Host Species: Rat
• Target Species: Mouse
• Gene ID (Entrez): 12504
• Primary or Secondary: Primary
• Product Type: Antibody