Invitrogen™ CD40L (Soluble) Mouse ELISA Kit

Sandwich ELISA Kit

Supplier:  Invitrogen™ BMS6010

Catalog No. 50-112-5440

Includes:

• Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse sCD40L
• Biotin-Conjugate anti-mouse sCD40L monoclonal antibody
• Streptavidin-HRP
• Mouse sCD40L Standard lyophilized, 40ng/mL upon reconstitution
• Sample Diluent
• Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
• Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
• Substrate Solution (tetramethyl-benzidine)
• Stop Solution (1M Phosphoric acid)
• Blue-Dye
• Green-Dye
• Red-Dye
• Adhesive Films

Description

The Mouse CD40 Ligand (Ms CD40L) ELISA quantitates Ms CD40L in mouse serum, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms CD40L. Principle of the method The Mouse CD40L solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD154 is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD154 stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD154 has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. Further, CD154 also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2. A defect in CD154 gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. CD154 is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. CD154 is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD154 also appears to be involved in the transduction of regulatory signals for cellular functions such as B cell proliferation and differentiation.

Specifications

• Accession Number: P27548
• Assay Sensitivity: 0.14 ng/mL
• Product Type: ELISA Kit
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CD154,CD40-L,Cd40l,HIGM1,IGM,IMD3,Ly-62,Ly62,T-BAM,TRAP,Tnfsf5,Tnlg8b,gp39
• Intraassay CV: 6.5%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 3 hr. 10 min.

• Assay Range: 0.31-20.0 ng/mL
• Conjugate: Biotin
• Sample Type: Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 21947
• Interassay CV: 11.1%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Sample Volume: Serum, 50 μL; Supernatant, 50 μL
• Synonym: TRAP, HIGM 1, T cell antigen Gp39, CD154
• Test Time: 1 hr. 20 min.

For Research Use Only