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CD41a, PerCP-eFluor™ 710, clone: HIP8, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 46041942

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Catalog No. 50-113-0748


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Description

Description

ITGA2B encodes integrin alpha chain 2b. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibronectin receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

Description: The HIP8 monoclonal antibody reacts with the human CD41 molecule, the integrin alpha IIb also known as platelet GPIIb. CD41 non-covalently associates with integrin beta3 (GPIIIa, CD61) and is expressed by megakaryocytes and platelets. The CD41/CD61 complex is a receptor for fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin and mediates platelets aggregation. HIP8 blocks platelet aggregation.Applications Reported: This HIP8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIP8 antibody has been pre-titrated and tested by flow cytometric analysis of human platelets. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2µm post-manufacturing filtered.
Specifications

Specifications

CD41a
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P08514
BDPLT2, CD41, CD41B, GP2B, GPIIb, GT, GTA, HPA3, GPalpha IIb, integrin alpha-IIb, platelet fibrinogen receptor, alpha subunit, platelet membrane glycoprotein IIb, platelet-specific antigen BAK, ITGA2B
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
HIP8
PerCP-eFluor 710
ITGA2B
Liquid
ITGA2B
IgG1, kappa, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
3674
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