CD42a Monoclonal Antibody (GR-P), eFluor™ 450, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-245-992	Invitrogen 48042841	25 Tests
50-245-993	Invitrogen 48042842	100 Tests

Description

Description: This GR-P monoclonal antibody reacts with human CD42a, which is also known as glycoprotein IX (GPIX). CD42a is a 22-kDa transmembrane protein that is constitutively expressed on platelets and megakaryocytes. CD42a associates with CD42b, CD42c, and CD42d to form the GPIb-V-IX complex, which binds the von Willebrand factor, an endothelial adhesion protein, to mediate platelet adhesion and activation. This binding results in platelet aggregation at sites of vascular injury. This receptor complex has also been demonstrated to bind thrombin, factors XI and XII, P-selectin, and Mac-1. Finally, a point mutation within the leucine-rich motif of CD42a has been linked to Bernard-Soulier syndrome. Applications Reported: Anti-Human CD42a eFluor™ 450 has been reported for use in flow cytometric analysis. Applications Tested: The GR-P antibody has been pre-titrated and tested by flow cytometric analysis of normal human platelets. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

CD42a Monoclonal antibody specifically detects CD42a in Human samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD42a
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Alias: CD42a; Glycoprotein 9; glycoprotein 9 (platelet); glycoprotein IX (platelet); glycoprotein IX platelet; GP9; GPIX; GP-IX; platelet glycoprotein IX; Platelet GPIX
• Gene Symbols: GP9
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: GP9

• Clone: GR-P
• Applications: Flow Cytometry
• Conjugate: eFluor 450
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: P14770
• Gene ID (Entrez): 2815
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.