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CD45.1 Monoclonal Antibody (A20), Super Bright™ 702, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Supplier: Invitrogen 67045382
Description
Description: The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells. Applications Reported: This UCHL1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This UCHL1 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (1 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
CD45.1 Monoclonal antibody specifically detects CD45.1 in Mouse samples. It is validated for Flow Cytometry.Specifications
CD45.1 | |
Monoclonal | |
0.2 mg/mL | |
PBS with BSA and 0.09% sodium azide; pH 7.2 | |
P06800 | |
Ptprc | |
Affinity chromatography | |
RUO | |
19264 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
A20 | |
Super Bright 702 | |
Ptprc | |
B220; Cd45; CD45 antigen; CD45R; GP180; Lca; L-CA; Leukocyte common antigen; loc; LY5; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen | |
Mouse | |
100 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2a κ |
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For Research Use Only