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CD45.2 Monoclonal Antibody (104), APC-eFluor™ 780, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Supplier: Invitrogen 47045480
Description
Description: The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. CD45.2 is expressed by all leukocytes in these strains. Applications Reported: This 104 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 104 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat.
CD45.2 Monoclonal antibody specifically detects CD45.2 in Mouse samples. It is validated for Flow Cytometry.Specifications
CD45.2 | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P06800 | |
Ptprc | |
Affinity chromatography | |
RUO | |
19264 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
104 | |
APC-eFluor 780 | |
Ptprc | |
B220; Cd45; CD45 antigen; CD45R; GP180; LCA; L-CA; leukocyte common antigen; loc; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; Ptprc; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein | |
Mouse | |
25 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2a κ |
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For Research Use Only.