CD45R (B220) Rat anti-Human, Mouse, eFluor® 570, Clone: RA3-6B2, eBioscience
Rat Monoclonal Antibody
Manufacturer: Invitrogen 41045280
Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen. Applications Reported: This RA3-6B2 antibody has been reported for use in microscopy, immunocytochemistry, and immunohistochemical staining of formalin-fixed paraffin embedded or frozen tissue sections. Applications Tested: This RA3-6B2 antibody has been tested by immunohistochemistry of frozen mouse spleen and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable. Excitation: 555 nm; Emission: 570 nmCD45R is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|B220, CD45R, Cd45, Ly-5, Lyt-4, T200, Cluster designation 45R|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)|
|4° C, store in dark, DO NOT FREEZE!|
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