CD45RA Monoclonal Antibody (HI100), NovaFluor™ Yellow 610, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
H014T02Y03	LIFE TECHNOLOGIES H014T02Y03	25 Tests
H014T03Y03	LIFE TECHNOLOGIES H014T03Y03	100 Tests

Description

The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral T lymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets. Applications Reported: The HI100 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HI100 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.8 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Each NovaFluor conjugate or kit is shipped with B001T06F01, CellBlox Blocking Buffer. Use 5 uL of CellBlox Blocking Buffer per stained cell sample containing 10^3 to 10^8 cells. Excitation: 552 nm; Emission: 612 nm; Laser: 561 nm (Yellow) Laser NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts.

CD45RA Monoclonal antibody specifically detects CD45RA in Human samples. It is validated for Flow Cytometry

Specifications

• Antigen: CD45RA
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: B220; CD45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; LCA; L-CA; leucocyte common antigen; Leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; Lyt-4; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; PTPRC; Receptor-type tyrosine-protein phosphatase C; RT7; T100; T200; T200 glycoprotein; T200 leukocyte common antigen
• Gene Symbols: PTPRC
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: PTPRC

• Clone: HI100
• Applications: Flow Cytometry
• Conjugate: NovaFluor Yellow 610
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: P08575
• Gene ID (Entrez): 5788
• Isotype: IgG2b κ
• Purification Method: Affinity chromatography
• Product Type: Antibody