Description: The monoclonal antibody miap301 reacts to CD47 also known as integrin-associated protein (IAP), and neurophilin. CD47 is a glycosylated five transmembrane protein with a small cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with ?3 integrins CD51/CD61, CD41/CD61. It is also known to be the receptor for thrombospondin. T cell expression of CD47 can mediate activation or apoptosis (in the presence of high levels of thrombospondin). Expression is found in the majority of hematopoietic cells including T and B cells, monocytes, platelets and erythrocytes (as part of the Rh complex). Expression is also found in non-hematopoietic cells. No crossreactivity is seen against human CD47. Applications Reported: This miap301 antibody has been reported for use in flow cytometric analysis and functional assays (blocking cell aggregation in vitro). Applications Tested: This miap301 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per million cells in a 100 µL total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC. CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes. CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.
|Flow Cytometry, Functional Assay, Neutralization|
|PBS with no preservative; pH 7.2|
For Research Use Only.
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