CD49b (Integrin alpha 2) Monoclonal Antibody (HMa2), eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-126-24	Invitrogen 14049182	100 μg

Description

Description: The HMalpha2 monoclonal antibody reacts with mouse CD49b, the 150 kDa integrin alpha2 subunit. The complex of CD49b non-covalently associated with integrin beta1 (CD29), also known as VLA-2, is a receptor for collagen and laminin. This complex is expressed by some CD4^+ T cells, IEL, NK cells, platelets and epithelial cells. Applications Reported: The HM alpha2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue (IHC-F). It has also been reported for use in in vitro functional assays (Please use Functional Grade purified HMa2, cat. 16-0491, in functional assays.). Applications Tested: The HM alpha2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

CD49b (Integrin alpha 2, Integrin alpha 2 chain, VLA-2 alpha chain, HM alpha 2) is a member of the integrin family. It is a glycoprotein with molecular weight of 150 kD, and it complexes with CD29 (Integrin beta 1) to form the heterodimeric integrin VLA-2 (integrin alpha 2 beta 1, or GPIa/IIa) complex. VLA-2 is an extracellular receptor for laminin, collagen, and fibronectin, and interaction with its ligands results in the activation of intracellular signaling pathways. It has reported roles in VEGF-induced angiogenesis in vivo, as well as adhesion and lymphocyte activation. CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, and epithelial cells. It is also expressed on adaptive immune cells such as T and B cells, specifically on a subset of CD4+ T cells in the spleen, on intraepithelial and lamina propria lymphocytes in the intestine, as well as on a population of peripheral CD4+ type 1 T regulatory (Tr1) cells that co-express LAG-3.

Specifications

• Antigen: CD49b (Integrin alpha 2)
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: Q62469
• Gene Symbols: Itga2
• Primary or Secondary: Primary
• Content And Storage: 4° C
• Gene: Itga2

• Clone: HMa2
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation
• Conjugate: Unconjugated
• Host Species: Armenian Hamster
• Target Species: Mouse
• Gene ID (Entrez): 16398
• Isotype: IgG
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.