CD5 Monoclonal Antibody (53-7.3), NovaFluor™ Blue 660-120S, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  LIFE TECHNOLOGIES M026T02B08

Catalog No. M026T02B08

Description

The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by a majority of thymocytes, mature T cells and a subset of B cells. The expression of CD5 by a small subset of B cells characterizes a developmentally and functionally distinct lineage of B cells called B-1 cells. CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction. Applications Reported: This 53-7.3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 53-7.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes. Use 5 μL of CellBlox Blocking Buffer per stained cell sample containing 10^3 to 10^8 cells. Excitation: 509 nm; Emission: 665 nm; Laser: 488 nm (Blue) Laser

CD5 Monoclonal antibody specifically detects CD5 in Mouse samples. It is validated for Flow Cytometry

Specifications

• Antigen: CD5
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P13379
• Host Species: Rat
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: 53-7.3
• Conjugate: NovaFluor Blue 660-120S
• Gene: Cd5
• Gene Alias: alanyl (membrane) aminopeptidase; alanyl aminopeptidase; alanyl aminopeptidase, membrane; aminopeptidase M; Aminopeptidase N; ANPEP; AP-M; APN; AP-N; CD antigen CD5; CD13; CD28; CD28 antigen (Tp44); CD28 molecule; Cd5; CD5 antigen; CD5 antigen (p56 62); CD5 antigen (p56-62); CD5 antigen p56-62; CD5 molecule; CD5 protein; CD74 antigen, invariant polypeptide of major; cell surface protein; costimulatory molecule B7 receptor CD28; fCD5; GP150; hAPN; LAP1; LEU1; Ly-1; Ly12; Ly-12; LyA; Ly-A; Lymphocyte antigen 1; Lymphocyte antigen CD5; lymphocyte antigen T1/Leu-1; Lyt-1; membrane alanyl aminopeptidase; microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; P150; Pan T cell; PEPN; T1; T-cell costimulatory molecule CD28; T-cell surface glycoprotein CD5; T-cell-specific surface glycoprotein CD28; unnamed protein product
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12507
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid