CD5 Mouse anti-Human, Clone: B-B8, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP247981B	Novus Biologicals NBP2479810.2MG	0.2 mg

Description

CD5 Monoclonal specifically detects CD5 in Human samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, CyTOF-ready.

Specifications

• Antigen: CD5
• Concentration: 1.0 mg/mL
• Applications: Flow Cytometry, Immunofluorescence, CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Adaptive Immunity, B Cell Development and Differentiation Markers, Cell Biology, Cytokine Research, Immunology
• Formulation: PBS with No Preservative
• Gene ID (Entrez): 921
• Immunogen: T cells activated with PHA
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 67 kDa

• Clone: B-B8
• Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: CD5 antigen, CD5 antigen (p56-62), CD5 molecule, LEU1T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, T1
• Gene Symbols: CD5
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes a 67kDa transmembrane protein which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (∼10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Note that this MAb is not suitable for frozen tissues.