CD5 Antibody (CD5/54/F6), Alexa Fluor™ 750, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP234593AF750

Catalog No. NP23493A70

Description

CD5 Monoclonal specifically detects CD5 in Human, Rat, Porcine, Bovine, Chicken, Equine, Guinea Pig, Monkey, Rabbit samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: CD5
• Classification: Monoclonal
• Conjugate: Alexa Fluor 750
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Symbols: CD5
• Immunogen: Recombinant fragment (around aa450-495) of human CD5 protein (intracellular region). (Uniprot: P06127 )
• Quantity: 0.1 mL
• Gene ID (Entrez): 921
• Target Species: Human, Rat, Porcine, Bovine, Canine, Chicken, Equine, Guinea Pig, Monkey, Rabbit
• Isotype: IgG1 κ

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: CD5/54/F6
• Dilution: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
• Gene Alias: CD5 antigen, CD5 antigen (p56-62), CD5 molecule, LEU1T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, T1
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Primary or Secondary: Primary
• Test Specificity: Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (∼10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.
• Content And Storage: Store at 4°C in the dark.