CD5 Monoclonal Antibody (YKIX322.3), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen 46505042

Catalog No. 50-160-58

Description

Description: This YKIX322.3 monoclonal antibody reacts with canine CD5, also known as Ly-1. CD5 is a 67-kDa type I transmembrane glycoprotein expressed on thymocytes and mature T cells. Studies have demonstrated that NK cells in dogs also express CD5, but at lower levels. In dogs, CD5 is not detectable on B cells or B cell lines. Applications Reported: This YKIX322.3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This YKIX322.3 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

CD5 Monoclonal antibody specifically detects CD5 in Canine samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD5
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: 0
• Gene Symbols: Cd5
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 612338
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: YKIX322.3
• Conjugate: PerCP-eFluor 710
• Gene: Cd5
• Gene Alias: alanyl (membrane) aminopeptidase; alanyl aminopeptidase; alanyl aminopeptidase, membrane; aminopeptidase M; Aminopeptidase N; ANPEP; AP-M; APN; AP-N; CD antigen CD5; CD13; CD28; CD28 antigen (Tp44); CD28 molecule; Cd5; CD5 antigen; CD5 antigen (p56 62); CD5 antigen (p56-62); CD5 antigen p56-62; CD5 molecule; CD5 protein; CD74 antigen, invariant polypeptide of major; cell surface protein; costimulatory molecule B7 receptor CD28; fCD5; GP150; hAPN; LAP1; LEU1; Ly-1; Ly12; Ly-12; LyA; Ly-A; Lymphocyte antigen 1; Lymphocyte antigen CD5; lymphocyte antigen T1/Leu-1; Lyt-1; membrane alanyl aminopeptidase; microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; P150; Pan T cell; PEPN; T1; T-cell costimulatory molecule CD28; T-cell surface glycoprotein CD5; T-cell-specific surface glycoprotein CD28; unnamed protein product
• Host Species: Rat
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Canine
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.