CD54 Mouse Anti-Rat, Brilliant Violet 650, Clone: 1A29, BD Optibuild™
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 742407
The 1A29 monoclonal antibody specifically recognizes ICAM-1 which is also known as CD54. ICAM-1 is an 85 kDa type I transmembrane glycoprotein that is encoded by Icam1 (Intercellular adhesion molecule 1). It is expressed on vascular endothelium in lymphoid tissues, thymic stromal cells, peripheral blood monocytes, peritoneal macrophages and mast cells, dendritic cells, and weakly on peripheral lymphocytes and thymocytes. ICAM-1 is a ligand for LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). Its expression is upregulated on activated lymphocytes and endothelial cells. 1A29 mAb inhibits Phorbol 12-Myristate 13-Acetate (PMA)-induced aggregation of phytohemagglutinin (PHA)-stimulated splenic blasts, as well as the adhesion of mitogen-stimulated blasts to high endothelial venule (HEV) cells and purified ICAM-1. The 1A29 antibody can reportedly inhibit leucocyte infiltration in in vivo systems, blocks induction of experimental allergic encephalomyelitis, and reduces NK-cell adhesion to tumor cells.The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|ICAM-1, Icam1, ICAM, Intercellular adhesion molecule 1|
|Rat HEV-Derived Cell Line Ax|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV650 under optimal conditions that minimize unconjugated dye and antibody.|
|Brilliant Violet 650|
|Mouse BALB/c IgG1, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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