CD64 Monoclonal Antibody (X54-5/7.1), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  eBioscience 46064180

Catalog No. 46-064-180

Description

Description: This X54-5/7.1 monoclonal antibody recognizes allelic variants a and b of mouse CD64, a high affinity Fc binding receptor also known as Fc-gammaR1 or Fcgr1. Alloform a is present in the following mouse strains: C57BL/6, BALB/c, DBA/2, and alloform b in strains: C3H/HeJ, CBA/J, NZW, SJL/J, 129/SvJ. In addition this antibody has been reported positive in strains: AKR, ALR, BUB, C58, CE, HRS,MRL, MON, NZB, NZO, NZW, PL, SJL, ST and SWR. This clone X54-5/7.1 will not recognize the allelic variant d, present in NOD/Lt mice. This antibody has been reported to stain RAW264.7 cell line. Applications Reported: This X54-5/7.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This X54-5/7.1 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative.

CD64 Monoclonal antibody specifically detects CD64 in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD64
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P26151
• Gene Symbols: Fcgr1
• Immunogen: BALB/c mouse CD64-human IgG Fc fusion protein
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Flow Cytometry
• Clone: X54-5/7.1
• Conjugate: PerCP-eFluor 710
• Gene: Fcgr1
• Gene Alias: AI323638; AV092959; CD64; CD64 antigen; CD64A; Fc fragment of IgG high affinity Ib receptor; Fc fragment of IgG receptor Ia; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for (CD64); Fc fragment of IgG, high affinity Ib, receptor for (CD64); Fc gamma receptor; Fc gamma receptor Ia; Fc receptor, IgG, high affinity I; Fcg1; Fc-gamma receptor 1; Fc-gamma receptor I A1; Fc-gamma RI; Fc-gamma RIA; FcgammaRI; FcgammaRIa; Fcgr1; FCGR1A; Fcgr1b; FcgRI; FCRI; FLJ18345; high affinity IgG Fc receptor, subunit beta; high affinity immunoglobulin gamma Fc receptor I; high-affinity immunoglobulin gamma Fc receptor I; IGFR1; IgG Fc receptor I; IgG high affinity Fc receptor; IGGHAFC; RP11-196G18.2
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 14129
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid