Description: This 815CU17 monoclonal antibody reacts with human CD68, a 110 kDa, transmembrane glycoprotein that belongs to the sialomucin family and is closely related to the lysosomal-associated membrane proteins (LAMP) and scavenger receptor. CD68 is predomitly an intracellular protein, found mainly in the late endosomal compartment but can be detected in smaller amounts on the surface of mainly myeloid-derived cells: monocytes, macrophages, dendritic cells (and in some langerhans cells), neutrophils, basophils, mast cells, myeloid progenitor cells, and a subset of CD34+ hematopoietic bone marrow progenitor cells. The function has not been fully elucidated but based on homology and structure, CD68 may play a role in antigen processing or presentation and protection of the lysosomal membrane from hydrolytic enzymes. Reports have also shown expression in activated T cells, and about 40% of peripheral blood B lymphocytes and 50% of all B-ALL. This 815CU17 antibody does not cross-react with mouse or rat CD68. For flow cytometric analysis of human CD68, we recommend clone eBioY1/82A. Applications Reported: This 815CU17 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.Applications Tested: This 815CU17 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 µg/mL.The 815CU17 antibody has been tested by western blot analysis of reduced PBMC lysate and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predomitly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human maligcies, including several Acute Myeloid Leukemia studies.
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot|
|PBS with 0.09% sodium azide; pH 7.2|
For Research Use Only.
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