CD68 Rat anti-Mouse, eFluor® 660, Clone: FA-11, eBioscience
Rat Monoclonal Antibody
Manufacturer: Invitrogen 50068180
Description: This FA-11 monoclonal antibody reacts with mouse CD68, known also as macrosialin. CD68 is a heavily O- and N- glycosylated type I integral membrane protein belonging to the sialomucin family and is closely related to the Lysosomal/endosomal-Associated Membrane Glycoproteins (LAMP). Although commonly used as a pan-macrophage marker, low levels of expression have also been observed in Langerhans cells and conventional dendritic cells. CD68 is mostly localized in the late endosomes and lysosomes with a smaller fraction circulating to the cell surface. In the steady state, only about 10-15% of CD68 is present at the surface. Cell stimulation increases expression of CD68, its transport to cell surface, and results in glycoform remodeling. CD68 binds oxidized LDL and is involved in the process of phagocytosis. The FA-11 antibody is not recommended for use in immunohistochemistry of formalin-fixed paraffin embedded tissues. Applications Reported: This FA-11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. Applications Tested: This FA-11 antibody has been tested by intracellular staining and flow cytometric analysis of mouse bone marrow-derived macrophages using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol.Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This FA-11 antibody has been tested using frozen mouse tissue and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered. CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predomitly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human maligcies, including several Acute Myeloid Leukemia studies.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|4° C, store in dark, DO NOT FREEZE!|
|Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)|
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