CD68 Monoclonal Antibody (FA-11), PE-Cyanine7, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen 25068180

Catalog No. 50-112-3301

Description

Description: This FA-11 monoclonal antibody reacts with mouse CD68, known also as macrosialin. CD68 is a heavily O- and N- glycosylated type I integral membrane protein belonging to the sialomucin family and is closely related to the Lysosomal/endosomal-Associated Membrane Glycoproteins (LAMP). Although commonly used as a pan-macrophage marker, low levels of expression have also been observed in Langerhans cells and conventional dendritic cells. CD68 is mostly localized in the late endosomes and lysosomes with a smaller fraction circulating to the cell surface. In the steady state, only about 10-15% of CD68 is present at the surface. Cell stimulation increases expression of CD68, its transport to cell surface, and results in glycoform remodeling. CD68 binds oxidized LDL and is involved in the process of phagocytosis. The FA-11 antibody is not recommended for use in immunohistochemistry of formalin-fixed paraffin embedded tissues. Applications Reported: This FA-11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This FA-11 antibody has been tested by intracellular staining and flow cytometric analysis of mouse bone marrow-derived macrophages using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online.

CD68 Monoclonal antibody specifically detects CD68 in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD68
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P31996
• Gene Symbols: Cd68
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12514
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: FA-11
• Conjugate: PE-Cyanine7
• Gene: Cd68
• Gene Alias: CD68; CD68 antigen; CD68 molecule; GP110; Lamp4; macrophage antigen CD68; macrosialin; Scard1; scavenger receptor class D, member 1; similar to CD68 antigen
• Host Species: Rat
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.