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CD69 Monoclonal Antibody (H1.2F3), eFluor™ 450, eBioscience™, Invitrogen™
Armenian Hamster Monoclonal Antibody
Supplier: Invitrogen 48069182
Description
Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation. Applications Reported: This H1.2F3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This H1.2F3 antibody has been tested by flow cytometric analysis of 24 hour Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered.
CD69 Monoclonal antibody specifically detects CD69 in Mouse samples. It is validated for Flow Cytometry.Specifications
CD69 | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P37217 | |
CD69 | |
Affinity chromatography | |
RUO | |
12515 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
H1.2F3 | |
eFluor 450 | |
CD69 | |
5830438K24Rik; Activation inducer molecule; activation inducer molecule (AIM/CD69); AI452015; AIM; BL-AC/P26; CD69; CD69 antigen; CD69 antigen (p60, early T-cell activation antigen); CD69 molecule; CLEC2C; C-type lectin domain family 2 member C; C-type lectin domain family 2, member C; EA1; early activation antigen CD69; early lymphocyte activation antigen; early T-cell activation antigen p60; GP32/28; leukocyte surface antigen Leu-23; MLR-3; VEA; Very Early Activation Antigen | |
Armenian Hamster | |
100 μg | |
Primary | |
Mouse | |
Antibody | |
IgG |
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For Research Use Only.