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CD72 Mouse anti-Human, eFluor® 660, Clone: J4-117, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 50072942

Catalog No. 50-112-4284


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Description

Description

Description: The monoclonal antibody J4-117 reacts with human CD72, a 45 kDa type II transmembrane glycoprotein that belongs to the C-type lectin superfamily. This glycoprotein is expressed on B cells at all stages of maturation except for plasma cells. CD72 is also expressed on dendritic cells, mast cells, and some macrophages. This receptor contains an immune-receptor tyrosine-base inhibition motif (ITIM) that associates with a tyrosine phosphatase, SHP-1. CD72 negatively regulates the major signal transduction pathways activated by the B cell receptor (BCR). Studies have demonstrated that the ligand for CD72 is CD100 (also known as Semaphorin 4D). CD72 can also react with CD5 to provide signaling between T and B cells. Finally, this receptor also prevents naive B cells from producing Ig and differentiating into B cells, and plays a role in controlling autoimmunity by enhancing apoptosis after Ag-specific BCR stimulation. Applications Reported: This J4-117 antibody has been reported for use in flow cytometric analysis. Applications Tested: This J4-117 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered. CD72 is a transmembrane glycoprotein expressed as a homodimer especially in B cells, but also in other antigen presenting cells such as dendritic cells and macrophages. Through one of its immunoreceptor tyrosine-based inhibitory motives (ITIMs), CD72 interacts with tyrosine phosphatase SHP-1, thereby suppressing B cell responsiveness. Binding of CD72 with its ligand CD100 (Sema4D) prevents BCR association and phosphorylation of CD72 and results in dissociation of SHP-1 from CD72, thus enables B cell activation.
Specifications

Specifications

CD72
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P21854
RP11-331F9.9, CD72b, LYB2
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
J4-117
eFluor™ 660
CD72
Liquid
CD72
IgG2b, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
971
Documents
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