CD73 Monoclonal Antibody (eBioTY/11.8 (TY/11.8)), PE-Cyanine7, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen 25073180

Catalog No. 50-245-731

Description

Description: eBioTY/11.8 recognizes the with CD73 a 69-kDa GPI-anchored cell-surface protein with ecto-5'-nucleotidase activity. Expression on myeloid cells (CD11b) is restricted to the bone marrow. In human CD73 can be induced to secrete a soluble form with IL-2 suggesting a role in mediating activation signals. Differences between human and mouse CD73 have been reported. BALB/c mice have more CD4+CD73+ than CD8+CD73+ while the reciprocal is documented in humans. CD73 is expressed on a subset of lymphocytes and increases during lymphocyte maturation. Recently it has been found that memory CD4 T cells express and are similar to the uncommitted primed precursor helper cells (Thpp) that can differentiate into Th1 or Th2 cells. Furthermore CD73 has been found on regulatory T cells. Applications Reported: This eBioTY/11.8 (TY/11.8) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioTY/11.8 (TY/11.8) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

CD73 Monoclonal antibody specifically detects CD73 in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD73
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q61503
• Gene Symbols: NT5E
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 23959
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: eBioTY/11.8 (TY/11.8)
• Conjugate: PE-Cyanine7
• Gene: NT5E
• Gene Alias: 2210401F01Rik; 5 nucleotidase, ecto; 5' nucleotidase, ecto; 5'-NT; 5'-nucleotidase; 5'-nucleotidase ecto; 5'-nucleotidase, ecto (CD73); AI447961; CALJA; CD73; E5NT; ecto-5'-nucleotidase; eN; eNT; NT; Nt5; Nt5e; NTE; Purine 5-Prime-Nucleotidase
• Host Species: Rat
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG1

For Research Use Only.