CD8 Monoclonal Antibody (12.C7), FITC, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA183219

Catalog No. PIMA183219

Description

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL. Mouse anti Rabbit CD8 antibody, clone 12.C7 recognizes the rabbit CD8 cell surface antigen, expressed by a subset of T lymphocytes with cytotoxic/suppressor activity.

CD8 (Cluster of Differentiation 8) is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediate efficient cell-cell interactions within the immune system. The CD8 antigen acts as a co-receptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 is found on a T cell subset of normal cytotoxic/suppressor cells which make up approximately 20-35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer (NK) cells, subpopulations of peripheral blood null cells, thymocytes and bone marrow cells. The CD8 co-receptor functions as either a homodimer composed of two alpha chains, or as a disulfide-linked heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. The majority of CD8+ T cells express CD8 as a alpha/beta heterodimer. In HIV, the HIV-2 envelope glycoprotein binds CD8 alpha chain (but not the beta chain).

Specifications

• Antigen: CD8
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Gene: CD8A
• Gene Symbols: CD8A
• Immunogen: Proprietary Immunogen.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Rabbit
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry
• Clone: 12.C7
• Conjugate: FITC
• Gene Alias: CD8, Leu-2, MAL, p32, CD8a, CD8b, CD8alpha, CD8beta
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 100356269
• Content And Storage: 4°C or -20°C if preferred, store in dark
• Form: Liquid